The Enzymatic Decolorization of Textile Dyes by the Immobilized Polyphenol Oxidase from Quince Leaves

نویسندگان

  • Gulnur Arabaci
  • Ayse Usluoglu
چکیده

Water pollution due to release of industrial wastewater has already become a serious problem in almost every industry using dyes to color its products. In this work, polyphenol oxidase enzyme from quince (Cydonia Oblonga) leaves immobilized on calcium alginate beads was used for the successful and effective decolorization of textile industrial effluent. Polyphenol oxidase (PPO) enzyme was extracted from quince (Cydonia Oblonga) leaves and immobilized on calcium alginate beads. The kinetic properties of free and immobilized PPO were determined. Quince leaf PPO enzyme stability was increased after immobilization. The immobilized and free enzymes were employed for the decolorization of textile dyes. The dye solutions were prepared in the concentration of 100 mg/L in distilled water and incubated with free and immobilized quince (Cydonia Oblonga) leaf PPO for one hour. The percent decolorization was calculated by taking untreated dye solution. Immobilized PPO was significantly more effective in decolorizing the dyes as compared to free enzyme. Our results showed that the immobilized quince leaf PPO enzyme could be efficiently used for the removal of synthetic dyes from industrial effluents.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Sandal reactive dyes decolorization and cytotoxicity reduction using manganese peroxidase immobilized onto polyvinyl alcohol-alginate beads

BACKGROUND Fungal manganese peroxidases (MnPs) have great potential as bio-remediating agents and can be used continuously in the immobilized form like many other enzymes. RESULTS In the present study, purified manganese peroxidase (MnP) enzyme isolated from Ganoderma lucidum IBL-05 was immobilized onto polyvinyl alcohol-alginate beads and investigated its potential for the decolorization and...

متن کامل

Decolorization and detoxification of textile dyes with a laccase from Trametes hirsuta.

Trametes hirsuta and a purified laccase from this organism were able to degrade triarylmethane, indigoid, azo, and anthraquinonic dyes. Initial decolorization velocities depended on the substituents on the phenolic rings of the dyes. Immobilization of the T. hirsuta laccase on alumina enhanced the thermal stabilities of the enzyme and its tolerance against some enzyme inhibitors, such as halide...

متن کامل

Decolorization of Cationic and Anionic Textile Blue Dyes from Aqueous Solution with Advanced Oxidation Process Using H2O2 and Various Catalysts

In this study, we used advanced oxidation process (AOP)for the removal of a cationic and anionic blue dyes, namely Basic Blue 3 (BB3) and Acid blue 62(AB62), from aqueous solutions. Fenton reactions are mixture of H2 O2 and Fe2+. However this paper investigates the application of twelve different catalysts such as: FeCl2 , FeSO4, (NH4 )Fe(SO4 )2 , FeCl3 , Nano iron (Fe0 ), Fe(NO3 )3 , K2FeO4 , ...

متن کامل

Decolorization of Ionic Dyes from Synthesized Textile Wastewater by Nanofiltration Using Response Surface Methodology

Decolorization of aqueous solutions containing ionic dyes (Reactive Blue 19 and Acid Black 172) by a TFC commercial polyamide nanofilter (NF) in a spiral wound configuration was studied. The effect of operating parameters including feed concentration (60-180 mg/l), pressure (0.5-1.1 MPa) and pH (6-10) on dye removal efficiency was evaluated. The response surface method (RSM) was utilized for th...

متن کامل

حذف رنگ مستقیم سبز 6 با استفاده از نشانده شدن آنزیم لاکاس بر روی نانو ذرات فریت روی از محلول های آبی

Background and Objective: Manufactured wastewater management of industrial units containing toxic pollutants is essential for environmental protection. Considering the great applications and effects of using the nanomaterial and nanotechnology in the field of environmental protection, the nanoparticle of ZnFe2O4 has been used as a basic particle. On the other hand, enzyme processes, due to thei...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره 2014  شماره 

صفحات  -

تاریخ انتشار 2014